Otomyiasis: Systematic Review

Abstract Introduction Myiasis is a disease causedby fly larvae that growinthetissues of animals and humans. It can cause a variety of local symptoms, like erythema or pain, depending on its location, and generalized symptomatology, such as fever andmalaise.Myiasis can generate severe complications, for instance sepsis, or directly impact vital tissues. Its management varies depending on the location, and on the preferences of the doctor that faces this challenge. Myiasis usually occurs in tropical countries, and, in many places, it is not a rare condition. The cases are rarely reported, and there are no publishedmanagement protocols. Objective To review the literature regarding the most common agents, the predisposing factors and the treatment alternatives for otic myiasis, a rare form of human myiasis caused by the infestation of fly larvae in the ear cavities. Data synthesis We present a systematic review of the literature. The search in five databases (Medline, Embase, Cochrane Database of Systematic Reviews, LILACS and RedALyC) led to 63 published cases from 24 countries, in the 5 continents. The ages of the patients ranged fromnewborn to 65 years old. Themost common agents belong to the Sarcophagidae or Calliphoridae families. Chronic otitis media, previous otic surgical procedures, mental deficit, alcohol or drug abuse, sleeping outdoors, prostration, and malnutrition were predisposing factors. The treatment alternatives are herein discussed. Conclusion The results highlight the need for monitoring, follow-up and standardization of medical approaches. (AU)

Molecular identification of five common Sarcophagidae species of necrophagous flies from Luoyang / 中国法医学杂志

Objective To identify the common Sarcophagidae species of necrophagous flies in Luoyang by DNA barcoding and 28S ribosomal RNA(28S rRNA) gene and evaluate its effectiveness for forensic practice. Methods Eighteen Sarcosaprophagous flies were collected and classified by entomologists with traditional morphological characteristics. The DNA of flies was extracted with Chelex-100 method. The fragments of mitochondrial cytochromec oxidase subunit I (COI) and 28S rRNA gene were amplified and sequenced. Twenty corresponding species (China and South Korea) were loaded from Barcode of Life Data System (BOLD) and added to the alignment. All the sequences were analyzed by MEGA 7.0 software package for nucleotide composition, genetic distance computation and phylogenetic tree construction. Results Eighteen Sarcosaprophagous flies were classified into 5 species of 3 genera. The result of amplification with 18 samples showed that length of the obtained COI and 28S rRNA gene sequences were 646bp and 721bp, respectively. And the result of alignment on BLAST online showed that index of similarity of the same species was above 99%. The thirty-eight COI sequences of Sarcosaprophagous flies were clustered into five groups by a neighbor-joining (NJ) tree on value of Bootstrap 1000. The intraspecific difference in COI was 0 to 0.022 while the interspecific difference ranged from 0.057 to 0.090 excluding Sarcophaga Africa and Sarcophaga haemorrhoidalis, which was 0~0.086. The NJ tree of 28S rRNA showed Sarcophaga peregrine and Sarcophaga portschinskyi sequences were obviously clustered into two groups and the others a group. Conclusion For the five sarcophagous flies in this study, the DNA barcoding based on COI gene were able to effectively identify the Sarcophaga peregrine, Sarcophaga dux and Sarcophaga portschinskyi, while 28S rRNA gene can only differentiate Sarcophaga peregrine from others. DNA barcoding based on COI gene and 28S rRNA gene can be used as supplemental molecular markers for identifying these species.

Sarchophagid flies (Insecta, Diptera) from pig carcasses in Minas Gerais, Brazil, with nine new records from the Cerrado, a threatened Neotropical biome

Sarchophagid flies (Insecta, Diptera) from pig carcasses in Minas Gerais, Brazil, with nine new records from the Cerrado, a threatened Neotropical biome. The diversity of the Sarcophagidae fauna of the Cerrado biome, also know as the Brazilian Savanna, is still underestimated. In this research we collected flies in the state of Minas Gerais, Brazil, during a Forensic Entomology experiment. Samples were collected throughout the decomposition process of domestic pig (Sus scrofa Linnaeus) carcasses, and the experiments were conducted in areas of pasture and semideciduous forest. A total of 85,694 adult flesh flies belonging to 57 species were collected from all carcasses. New records for nine species of Sarcophaginae are provided, including the first record of Blaesoxipha (Acridiophaga) caridei (Brèthes, 1906) to Brazil, and new occurrences of the following species for the Cerrado and/or for the state of Minas Gerais: Blaesoxipha (Acanthodotheca) acridiophagoides (Lopes & Downs, 1951), Malacophagomyia filamenta (Dodge, 1964), Nephochaetopteryx orbitalis (Curran & Walley, 1934), Nephochaetopteryx cyaneiventris Lopes, 1936, Nephochaetopteryx pallidiventris Townsend, 1934, Oxysarcodexia occulta Lopes, 1946, Ravinia effrenata (Walker, 1861) and Sarcophaga (Neobellieria) polistensis (Hall, 1933).

Two new Neotropical species of the genus Oxysarcodexia Townsend (Diptera, Sarcophagidae) / Duas novas espécies neotropicais do gênero Oxysarcodexia Townsend (Diptera, Sarcophagidae)

Two new Neotropical species of the genus Oxysarcodexia Townsend (Diptera, Sarcophagidae). Oxysarcodexia nitida sp. nov. and O. notata sp. nov. from Peru are described and illustrated based on male specimens. These new taxa are morphologically more similar to O. vittata (Walker) and O. xon (Dodge) respectively.

Oxysarcodexia nitida sp. nov. e O. notata sp. nov. do Peru são descritas e ilustradas com base em espécimes machos. Esses novos táxons são morfologicamente mais similares a O. vittata (Walker) e O. xon (Dodge), respectivamente.

Parasitoides larva-pupa de Hylesia metabus Cramer (Lepidoptera: Saturniidae) en la región nororiental de Venezuela: un caso de control Biológico Natural / Larvae-pupae parasitoids of Hylesia metabus Cramer (Lepidoptera: Saturniidae) in northeastern Venezuela: a case of natural biological control

Hylesia metabus Cramer es una polilla considerada un problema de salud pública debido a que las hembras adultas liberan setas urticantes produciendo dermatitis y prurito intenso en el hombre. En la región nororiental de Venezuela, en dos localidades (Mapire y Pedernales) y durante seis ciclos, se registró el parasitismo natural sobre esta polilla. Para ello, se colectaron pupas en el campo y se mantuvieron bajo condiciones de laboratorio (27ºC, 65 por ciento humedad relativa y ciclo invertido 12L:12O) hasta la eclosión de los adultos. Se determinó que en Mapire el parasitismo aumentó de 33,0 por ciento a 91,1 por ciento en tres ciclos consecutivos estudiados y los principales parasitoides fueron los Diptera: Belvosia spp. (Tachinidae) y Sarcodexia lambens (Sarcophagidae) y diversas especies de Hymenoptera (Ichneumonidae, Chalcidae, Perilampidae, Eulophidae). En Pedernales en tres ciclos consecutivos el parasitismo disminuyó de 29,5 por ciento a 16,1 por ciento, el parasitoide más abundante fue Belvosia spp., con niveles de parasitismo entre 70,7 por ciento y 96,2 por ciento. Solamente durante el tercer ciclo, la contribución de otro parasitoide resultó significativa y correspondió a Neotheronia sp. (Hymenoptera: Ichneumonidae), que causó 27,1 por ciento de parasitismo. Todos los parasitoides obtenidos fueron koinobiontes larva-pupa. Las variaciones poblacionales de H. metabus y sus parasitoides en las áreas estudiadas son discutidas. Belvosia spp. y Sarcodexia lambens son potenciales parasitoides a ser usados en programas de manejo integrado de este insecto plaga.

The moth Hylesia metabus Cramer is considered a public health pest due to the release of urticating hairs by adult females during periodic invasions of towns and villages, producing dermatits in humans. We reported natural parasitism levels for this moth during six life cycles in two localities in northeastern Venezuela. Pupae were collected in the field and maintained under laboratory conditions (27ºC, 65 percent RH, 12L:12D photoperiod) until eclosion. In Mapire, parasitism increased from 33.0 percent to 91.1 percent in the three cycles studied, with the dipterans Belvosia spp. (Tachinidae) and Sarcodexia lambens Wiedemann (Sarcophagidae) and species from Ichneumonidae, Chalcidae, Perilampidae and Eulophidae being the most common. In Pedernales, parasitism decreased from 29.5 percent to 16.1 percent, and Belvosia spp. was the most abundant parasitoids, reaching parasitization levels between 70.7 percent and 96.2 percent. A significative parasitization by Neotheronia sp. (Hymenoptera: Ichneumonidae) (27.1 percent) occurred only in third life cycle. All natural enemies observed were koinobionts, larval-pupa parasitoids. The variations in the populations of H. metabus and its parasitoids in the areas studied are discussed. Belvosia spp. and S. lambens may have potential for use in integrated management program of H. metabus.

Validação da armadilha adultrap® para capturade dípteros muscoides / Validation of the adultrap® trap for capture of muscoids dipterans

O potencial da armadilha adultrap® para captura de dípteros muscoides foi avaliado por comparação com a armadilha descrita por Ferreira (1978) e adaptada por Fraga e d' Almeida (2005). Iscas de sardinha foram utilizadas em ambas as armadilhas. O teste de Wilcoxon para duas amostras não pareadas foi empregado para comparação das armadilhas. Capturou-se um total de 8.909 dípteros muscoides, sendo 57,51 por cento desses pela adultrap®, mas sem diferença significativa (p = 0,147) entre as armadilhas. Do total de sarcofagídeos, califorídeos, muscídeos e de outras famílias de dípteros muscoides 79, 68, 29 e 31 por cento, respectivamente, foram capturados pela adultrap® com diferença estatística (p = 0,002) entre as armadilhas apenas para a família Sarcophagidae. Os resultados da adultrap® foram similares ao modelo modificado por Fraga e d' Almeida (2005). Entretanto, adultrap® foi mais eficiente na captura de dípteros da família Sarcophagidae.

The potential of adultrap® for capture of muscoid dipterans was evaluated by comparison with the trap described by Ferreira (1978) and adapted by Fraga and d' Almeida (2005). Sardine baits were used in both traps. The Wilcoxon two-sample test was used for trap comparison. A total of 8,909 muscoid dipterans was caught, 57.51 percent of them by the adultrap®. However, there was no significant difference (p = 0.147) between trap efficiency. From the total of sarcophagids, calliphorids, muscids and other families of muscoid flies, 79, 68, 29 and 31 percent were caught by adultrap®, respectively. Statistical difference (p = 0.002) between traps was found only for Sarcophagidae. The results of adultrap® were similar to the model used by Fraga and d'Almeida (2005), thus validating its use for seasonal fluctuation studies of muscoid dipterans. However, adultrap® has the highest efficiency in capturing Sarcophagidae flies.

Vejigas animales como sitios de oviposición y desarrollo de moscas en festividades, Heredia, Costa Rica / Animal bladders as oviposition and breeding sites for flies after festivities in Heredia, Costa Rica

Se realizó un recuento del númeo de vejigas dispersas en 15 manzanas del centro urbano de Barva, Heredia, luego de la celebración de las mascaradas, actividad enmarcada en las fiestas patronales de la ciudad. Adicionalmente se llevó a cabo una evaluación de los huevos, larvas y adultos de mosca presentes en 2 vejigas que fueron descartadas en lugares públicos y que no fueron contaminadas con tierra, fluidos u otros detritos. Luego de la actividad se contaron 29 vejigas, las cuales estuvieron localizadas en tendido eléctrico, 55.2 por ciento, aceras, 27,6 por ciento, jardines 6.9 por ciento, desagues 6.9 por ciento y techos 3.4 por ciento. Los grupos de moscas observados pertenecieron a las familias Calliphoridae, cochliomyia macellaria, lucilia eximia, phaenicia cuprina, chrysomyia megacephala, sarcophagidae, sarcophaga sp., muscidae, musca domestica y phoridae. Los promedios de huevos y larvas por vejiga fueron 176 y 44 respectivamente. Los resultados demuestran que las vejigas crudas constituyen un eficiente atrayente para diversas especies de moscas de importancia en salud pública, lo que plantea la necesidad de buscar alternativas de tratamiento para las mismas con el fin de garantizar la sostenibilidad de la tradición minimizando la contaminación del entorno urbano.

A study of the number of animal bladders dispersed in 15 blocks in the urban core of Barva- Heredia was performed following the masquerades, a traditional celebration during the festivities of the city. Additionally, an evaluation of the eggs, larvae and adult flies was performed in two bladders that were discarded in public areas, which were free from soil, fluids, or other debris. In total, 29 bladders were counted. They were located on power lines (55,2%), sidewalks (27,6%), drainages (6,9%) and roofs (3,4%). The flies observed corresponded to the families Calliphoridae (Cochliomyia macellaria, Lucilia eximia, Phaenicia cuprina, Chrysomyia megacephala), Sarcophagidae (Sarcophaga sp.), Muscidae (Musca domestica), and Phoridae. The average of eggs and larvae per bladder was 176 and 44, respectively. Results show that fresh bladders are an efficient bait for flies of medical importance. In this sense, there is a need to identify treatment methods for the bladders in order to guarantee sustainability of the radition, without the contamination of the urban...

Flesh flies species (Diptera: Sarcophagidae) from a grassland and a woodland in a Nature Reserve of Buenos Aires, Argentina

Temporal changes of flesh flies abundance were simultaneously recorded at monthly intervals during a year in a woodland and in a grassland at the "Reserva Ecológica Costanera Sur" (RECS), Buenos Aires city, Argentina. Samples were taken at monthly intervals (February 2004-January 2005). Hourly captures of adult flies (10:00 am - 04:00 pm) were taken each sampling date with a hand net. Temperature was recorded at each hourly capture. The baits used were 200 g of rotten cow liver and 200 g of fresh dog faeces. Records of abundance included only species whose abundance was ≥30 individuals accumulated during the whole sampling period. Considering overall abundance, a non-parametric Chi-square test was used to estimate deviations of an expected habitat and bait preference ratios of 1:1. The same criterion was applied to include species in a contingency table to describe their seasonality. The final matrix included four species whose associations to seasons were analyzed by using a Correspondence Analysis. To normalize the data, a log 10(n+1) transformation was applied prior to the analysis of correlation. Pearson product-moment correlations were used to examine the relationship between flesh-fly abundance with temperature and habitat preference. The entire sample accumulated 1 305 individuals and 18 species. The flies were more abundant in the grassland than in the woodland. Microcerella muehni, Oxysarcodexia paulistanensis, O. varia and Tricharaea (Sarcophagula) occidua exhibited the higher relative abundance, representing the remaining species less than 8 % of the entire sample. Most of the recorded species showed preference for faces as bait. Concerning the dominant species, all of them but M. muehni, a suggestively termophobic species, prevailed in late spring-summer. The observed species arrangement at both sites indicates low species diversity and equitability and high information per individual in the average. The referred community traits would indicate the possible effects of environmental disturbance due to the proximity of study sites to dense urban settlements. Rev. Biol. Trop. 56 (3): 1287-1294. Epub 2008 September 30.

Variaciones numéricas de especies de Sarcophagidae (Diptera) se registraron durante un año a intervalos mensuales (febrero 2004-enero 2005), en un pastizal y un bosque de la "Reserva Ecológica Costanera Sur" (RECS) de la ciudad de Buenos Aires. Se realizaron capturas horarias (10:00 am - 04:00 pm) en cada fecha de muestreo. La muestra de todo el año acumuló 1 305 individuos y 18 especies. Estos dípteros fueron más abundantes en el pastizal que en el bosque. Las especies más numerosas fueron: Microcerella muehni, Oxysarcodexia paulistanensis,O. varia y Tricharaea (Sarcophagula) occidua, en tanto que las restantes representaron menos de 8 % del total. La mayoría de las especies mostraron preferencia por las heces como cebo. Las especies dominantes, con excepción de la especie termofóbica M. muehni, prevalecieron en la primavera tardía y en el verano. La alta dominancia específica observada podría ser indicativa del desorden ecológico provocado por la influencia de asentamientos urbanos adyacentes a los sitios de muestreo.

Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva

We report for the first time the expression of multiple protease activities in the first instar larva (L1) of the flesh fly Oxysarcodexia thornax (Walker). Zymographic analysis of homogenates from freshly obtained L1 revealed a complex proteolytic profile ranging from 21.5 to 136 kDa. Although some activities were detected at pH 3.5 and 5.5, the optimum pH for most of the proteolytic activities was between pH 7.5 and 9.5. Seven of 10 proteases were completely inactivated by phenyl-methyl sulfonyl-fluoride, suggesting that main proteases expressed by L1 belong to serine proteases class. Complete inactivation of all enzymatic activities was obtained using N-p-Tosyl-L-phenylalanine chloromethyl ketone (100 µM), a specific inhibitor of chymotrypsin-like serine proteases.

Estudio de la entomofauna sucesional asociada a la descomposición de un cadáver de cerdo doméstico (Sus scrofa) en condiciones de campo / Study of successional entomofauna associated to domestic pig (Sus Scrofa) corpse decomposition in field conditions

Durante los meses de julio a septiembre de 2003, se realizó la caracterización de la entomofauna de importancia forense presente en la descomposición de dos cerdos (Sus scrofa), utilizados como sujeto de muestreo y control respectivamente, en la granja experimental de la Federación Nacional de Cafeteros de Colombia (sede Consacá, Nariño). Los primeros insectos colonizadores del cadáver fueron las familias Calliphoridae y Sarcophagidae (Diptera); de la primera se capturaron individuos pertenecientes a Chrysomya albiceps (Wiedemann 1819), Chrysomya rufifacies (Macquart 1843), Chrysomya putoria (Wiedemann 1818) y Lucilia sp. siendo C. albiceps y Lucilia sp. las más abundantes durante el muestreo. Además de Diptera se presentaron algunas familias del orden Coleoptera, de los cuales los más importantes fueron: Histeridae, Staphilinidae, Silphidae, como depredadores, y Dermestidae como necrófago. Los otros grupos de insectos fueron observados ocasionalmente. Sin embargo, Labidus sp. subfamilia Ecitoninae (Formicidae), que se comportó como depredador de larvas de Diptera, estuvo presente durante todo el proceso de descomposición.

During July to September of 2003, characterization of entomofauna attracted by decomposition of two pigs (Sus scrofa) was carried out in the experimental farm of Federación Nacional de Cafeteros de Colombia at Consacá (Nariño). One of the two pigs was used as a control sample. Pioneer settler insects of carcass belonged to families Calliphoridae and Sarcophagidae (Diptera). From Calliphoridae individuals from Chrysomya albiceps (Wiedemann 1819), Chrysomya putoria (Wiedemann 1818), Chrysomya rufifacies (Macquart 1843) and Lucilia sp. were captured. C. albiceps and Lucilia sp. were the most abundant species. Families from Coleoptera order were also found: Histeridae, Staphilinidae, Silphidae, as predators, and Dermestidae as necrophagous. Other groups of insects were observed occasionaly. Nevertheless, as predators of Diptera grubs, individuals of Labidus sp. (Formicidae: subfamily Ecitoninae) were present during the entire decomposition process.

Incidence of myiasis in Panama during the eradication of Cochliomyia hominivorax (Coquerel 1858, Diptera: Calliphoridae) (2002-2005)

We present the results of a study on myiasis in Panama during the first years of a Cochliomyia hominivorax eradication program (1998-2005), with the aim of investigating the behavior of the flies that produce myiasis in animals and human beings. The hosts that registered positive for myiasis were cattle (46.4 percent), dogs (15.3 percent), humans (14.7 percent), birds (12 percent), pigs (6 percent), horses (4 percent), and sheep (1 percent). Six fly species caused myiasis: Dermatobia hominis (58 percent), Phaenicia spp. (20 percent), Cochliomyia macellaria (19 percent), Chrysomya rufifacies (0.4 percent), and maggots of unidentified species belonging to the Sarcophagidae (3 percent) and Muscidae (0.3 percent). With the Dubois index, was no evidence that the absence of C. hominivorax allowed an increase in the cases of facultative myiasis.

Revisão das duas espécies de Tapacura Tibana & Lopes, 1985 (Diptera: Sarcophagidae: Sarcophaginae) / Revision of the two species of Tapacura Tibana & Lopes, 1985 (Diptera: Sarcophagidae: Sarcophaginae)

Esta revisão apresenta um estudo da morfologia externa das espécies de Tapacura Tibana & Lopes 1985, especialmente da terminália masculina. Tapacura inclui atualmente duas espécies: T. mariarum Tibana & Lopes, 1985 e T. mexicana Lopes, 1988, que foram relacionados a Peckiamyia Dodge, Retrocitomyia Lopes e a Sinopiella Lopes & Tibana na descrição original. São apresentadas redescrições, ilustrações e uma diagnose genérica mais ampla. Uma comparação entre as espécies de Tapacura e aquelas dos gêneros relacionados tem mostrado que Tapacura é mais similar morfologicamente a Peckiamyia, especialmente porque ambos têm o 3º setor costal da asa com pequenas cerdas ventrais, o fêmur médio do macho sem ctenídio e o edeago muito reduzido e pigmentado.

This revision presents a study on external morphology of the species of Tapacura Tibana & Lopes 1985, especially of the male terminalia. Tapacura includes presently two species: T. mariarum Tibana & Lopes, 1985 and T. mexicana Lopes, 1988, which were related to Peckiamyia Dodge, Retrocitomyia Lopes and Sinopiella Lopes & Tibana in the original description. Redescriptions, illustrations and a broadest generic diagnosis are presented. A comparison between the Tapacura species and those of the related genera has showed that Tapacura is more morphologically similar to Peckiamyia, especially because both have the third costal section of the wing with ventral setulae, male mid femur without ctenidium and a very reduced and pigmented aedeago.

Population dynamics of calyptrate diptera (Muscidae and Sarcophagidae) at the Rio-Zoo Foundation, Rio de Janeiro, RJ, Brazil

Twenty-seven species of calyptrate muscoids (Muscidae and Sarcophagidae) were collected from December 1993 to November 1994 with wind oriented traps (W.O.T.) baited with decomposing beef liver at the Rio de Janeiro Zoo. The most abundant species found were Musca domestica (57.84%), Peckia chrysostoma (28.16%), Ophyra aenescens (17.11%), Oxysarcodexia thornax (17.82%), Synthesiomyia nudiseta (13.05%), and Oxysarcodexia diana (14.52%).

Vinte e sete espécies de dípteros caliptrados (Muscidae e Sarcophagidae) foram capturados no período de dezembro de 1993 a novembro de 1994, com armadilhas orientadas pelo vento (W.O.T.) contendo isca de fígado em putrefação, no jardim zoológico da cidade do Rio de Janeiro. As espécies capturadas em maior abundância foram Musca domestica (57,84%), Peckia chrysostoma (28,16%), Ophyra aenescens (17,11%), Oxysarcodexia thornax (17,82%), Synthesiomyia nudiseta (13,05%) e Oxysarcodexia diana (14,52%).

Notes on species of the genus Agria R.-D. (Diptera, Sarcophagidae)

A key is given for the identification of females of all Holarctic species included into this genus, and keys are provided for distinguishing third instar larvae and puparia of two Palaeartic species. In addition to these, the description of the A. monachae (Kr.) female has been expanded.

Scanning eletron microscopy of the first instar larvae of Sarcodexia lambens and Peckia chrysostoma (Diptera: Sarcophagidae)

The first instar larvae of Sarcodexia lambens (Wiedemann, 1830) and Peckia chrysostoma (Wiedemann, 1830) dissected from females reared in laboratory, are described under scanning electron microscope (SEM).

Uma nova espécie de Farrimyia dodge, 1965 (Diptera, Sarcophagidae) / A new species of Farrimyia Dodge, 1965 (Diptera, Sarcophagidae)

The male of Farrimyia lopesi, a new species from Maracaí island, Uraricoera river, Roraima, Brazil is described and illustrated.

Sarcophagid flies (Diptera: Sarcophagidae) from Tripura, India

Systematic position of seventeen species under nine genera are reported here. Sinonipponia smarti is described as new species. Lioproctia (Coonoria) pattoni (Senior-White) is redescribed and figured.

A new species of Boettcheria (Diptera: Sarcophagidae) from the Dominican Republic

Boettcheria solo sp. n. from the Dominican Republic is described and it is argued that its most probable sister group is B. parkeri Aldrich, 1916 from Jamaica. Boettcheria Parker, 1914 is briefly diagnosed and a list of included species is provided.

Uma nova espécie de Punaphyto Townsend, 1916 (Diptera, Sarcophagidae) / A new species of Punaphyto Towsend, 1916 (Diptera, Sarcophagidae)

Male and female of Punaphyto lopesi, a new species from Equador are described and illustrated.

Prof. Hugo de Souza Lopes and the modern system of Sarcophagidae (Diptera)

Prof. Dr. Hugo de Souza Lopes is one of the authors of the phylogenetic classification of Sarcophagidae, especially Sarcophaginae. In this paper I present the taxonomic key of the tribes of Sarcophaginae according to his opinion; a list of the 48 genera and subgenera and the 356 species described by Prof. Lopes; and a review of subtribal construction of tribe Sarcophagini with a key of the subtribes. One new subtribe Boettcheriiscina Verves, subtr. nov. and two new monotypic genera (Mufindia Verves, gen. nov., and Sabiella Verves, gen. nov.) are described. The role of Prof. Lopes in the knowledge of taxonomy and ecology of American, Oriental, Australian and Oceanic Sarcophagidae is illumined.